Journal: International Journal of Molecular Sciences

Article Title: Novel Lipid Nanocomplex Co-Carrying Bcl2 siRNA and Quantum Dots for EGF Receptor-Targeted Anti-Cancer Theranosis

doi: 10.3390/ijms25116246

Figure Lengend Snippet: Tumor-targeted siRNA delivery of QDM and immuno-QDM. Protein expression of MDA-MB-453 (EGFR negative) and LS174T (EGFR positive) cells, and the target tumor cell binding and cellular uptake assay of Chol-siRNA, QDM, and immuno-QDM ( A – C ) were evaluated. All particles were incubated with the target cells (LS174T) for 1 h and 8 h at 37 °C and analyzed with a confocal microscope for evaluate siRNA delivery and endosomal escape analysis of QDM and immuno-QDM ( D ) (X400).

Article Snippet: The LS174T (human colorectal adenocarcinoma, #10188) cell line was purchased from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: Expressing, Binding Assay, Incubation, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Novel Lipid Nanocomplex Co-Carrying Bcl2 siRNA and Quantum Dots for EGF Receptor-Targeted Anti-Cancer Theranosis

doi: 10.3390/ijms25116246

Figure Lengend Snippet: Knockdown of target gene and in vitro anti-cancer efficacy of QDM/siBcl2 and iQDM/siBcl2. ( A , B ) The bcl2 gene knockdown was detected with PCR and Western blot (25, 50 pm). ( C ) MTT assay results of LS174T and MDA-MB-453 cells after the treatment of siRNA, QDM/siBcl2, and iQDM/siBcl2.

Article Snippet: The LS174T (human colorectal adenocarcinoma, #10188) cell line was purchased from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: Knockdown, In Vitro, Western Blot, MTT Assay

Journal: PLoS ONE

Article Title: Genetic Ablation of Afadin Causes Mislocalization and Deformation of Paneth Cells in the Mouse Small Intestinal Epithelium

doi: 10.1371/journal.pone.0110549

Figure Lengend Snippet: ( A ) Western blotting of afadin and EphB3 using immunoprecipitates with the indicated antibodies from the Ls174T colon cancer cell line. ( B ) Immunostaining of the small intestine of control and afadin -cKO mice with antibodies against EphB3 (green), lysozyme (red), and Hoechst33258 (blue). Scale bars = 50 µm. ( C ) Relative intensity of EphB3 signal in crypt cells to that of non-crypt cells is shown. 18 crypts from 6 pictures (control) and 24 crypts from 8 pictures ( afadin -cKO) were analyzed.

Article Snippet: The colon cancer cell line Ls174T (DS Pharma Biomedical Co., Osaka, Japan) was cultured in MEM containing 1% NEAA, 2 mM L-glutamine, and 10% FBS and lysed in 50 mM Tris HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% Nonidet P-40, 1 mM EGTA, and 10% glycerol supplemented with 1 μg/ml aprotinin, 1 μg/ml leupeptin, 20 μg/ml phenylmethylsulfonyl fluoride, and phosphatase inhibitors.

Techniques: Western Blot, Immunostaining, Control

Journal: PLoS ONE

Article Title: Krüppel-like Factor 4 Regulates Intestinal Epithelial Cell Morphology and Polarity

doi: 10.1371/journal.pone.0032492

Figure Lengend Snippet: (A) LS174 cells in 3D culture system were stained for differentiation markers indicating cell polarity and cyst formation. (B) Statistical analysis of percentage of cyst formation in LS174T cell 3D cultures, comparing between doxycycline (Dox)-induced and non-induced cells.(*, P<0.05) (C) Western blotting indicating expression of KLF4 in LS174T cell line with or without induction of doxycycline. (D) Semi-quantitative RT-PCR showing expression of genes related to KLF4-regulated cell polarity and related to Wnt signaling.

Article Snippet: LS174T colon cancer cell line was grown in RPMI medium (Mediatech) supplemented with 5% fetal bovine serum and 1% penicillin/streptomycin.

Techniques: Staining, Western Blot, Expressing, Quantitative RT-PCR